Genetika člověka
Mikrobiologické aplikace
NGS
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PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
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Real-time PCR cyclery
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Pipetovací roboty
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Jednokanálové
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PCR, qPCR, RT
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Reverzní transkripce
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Intolerance
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Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
Immortalized Human Tumor Fibroblasts (CAF) – SV40T
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Pancreas |
| Organism |
Human (H. sapiens) |
| Donor History |
Pancreatic cancer |
| Growth Properties |
Adherent ,fibroblast |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow III (TM003) + 20% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
The Immortalized Human Tumor Fibroblasts (CAF) – SV40T are a specialized cell line derived from cancer-associated fibroblasts (CAFs) and immortalized using the Simian Virus 40 large T antigen (SV40T). This immortalization enhances their proliferative capacity while preserving the phenotypic and functional characteristics of CAFs. These cells are an invaluable resource for investigating the role of fibroblasts in tumor progression, cancer-stromal interactions, and the tumor microenvironment. Applications include cancer research, drug discovery, and the study of therapeutic resistance mechanisms, providing a robust model for developing and testing novel anticancer therapies targeting the stromal components of tumors.
