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Biochemie
Imunoanalýza
Immortalized Mouse Astrocytes – SV40T (IMA2.1)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Brain |
| Organism |
Mouse (M. musculus) |
| Donor History |
Balb/c Mouse ,Cerebral Cortex |
| Growth Properties |
Adherent ,stellate shaped |
| Growth Conditions | |
| Immortalization Method |
Serial passaging and transfection with psV3neo plasmid carrying SV40 large T antigen genes |
| Full Information |
https://www.abmgood.com/immortalized-mouse-astrocytes-sv40t-ima2-1.html |
The Immortalized Mouse Astrocytes- SV40 T (IMA 2.1) is a suitable cell line for studies in astrocyte functions. This cell line is unique in that it is capable of metabolizing the parkinsonian toxin MPTP (to MPP+) and is responsive to pro-inflammatory stimuli. In addition, this cell line can be used in neuronal co-culture studies to simulate conditions in the brain.Upon stimulation with cytokines such as IL-1β, TNF-α and IFN-γ, these cells show up-regulated NOS-2 and COX-2 mRNA expression and release various inflammatory mediators. This cell line has a stable star-shape morphology and expresses immature astrocyte markers such as nestin and CD44, indicating that the cells are of the early-stage astrocyte precursor phenotype. Furthermore, they are capable of converting glutamate via glutamine synthase to glutamine, which is a characteristic of GFAP-negative astrocytes.
