Immortalized Mouse Dendritic Cells (MutuDC1940)

As messengers bridging the innate and adaptive immune systems, conventional/classical lymphoid tissue-resident dendritic cells (cDCs) capture and present antigens; acting as sentinels in secondary lymphoid organs and other tissues. The wild-type MutuDC1940 dendritic cell line is derived from mouse spleen tissues (CD11c:SV40LgT transgenic mice) and is a powerful tool for vaccine science and immunotherapy, particularly for strategizing target antigens to the CD8α+ subset (which has the CD11chigh, B220−, DEC205+, CD24high, CD11b− expression profile). MutuDC cell lines are GFP positive due to the GFP reporter in the CD11c:SV40LgT transgene. This cell line has the following characteristics:responds to TLR ligands such as CpG (TLR9-L), PolyIC (TLR3-L), and LPS (TLR4-L) by up-regulation of CD40, CD80 and CD86responds to PAMP stimulation by production of Th1 cytokines such as IL-12capable of MHC-I and MHC-II antigen-presentation (both direct and cross-presentation of cell-associated antigens; evaluated by by the MHC-I (SIINFEKL/OT-I ) and MHC-II (OVA323-339/OT-II) restricted systems)Additional MutuDC cell lines available at abm include:Cat. T3034 – TLR3 Knockout MutuDC Cell line Cat. T3035 – TLR9 Knockout MutuDC Cell line Cat. T3036 – Ifnar1 Knockout MutuDC Cell line