Genetika člověka
Mikrobiologické aplikace
NGS
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Real-time PCR cyclery
Fluorometry
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Izolátory nukleových kyselin
Pipetovací roboty
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Jednokanálové
Multikanálové
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PCR, qPCR, RT
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Reverzní transkripce
Alergie
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Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
Immortalized Mouse Intestinal Crypt Stem Cells (iMICs)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Intestine |
| Organism |
Mouse (M. musculus) |
| Donor History | |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions | |
| Immortalization Method |
Serial passaging and infection with retrovirus carrying loxP-flanked SV40 large T antigen |
| Full Information |
https://www.abmgood.com/immortalized-mouse-intestinal-crypt-stem-cells-imics.html |
Immortalized Mouse Intestinal Crypt Stem Cells (iMICs) are immortalized through infection with non-replicating retrovirus carrying the loxP-flanked SV40 large T antigen. This conditionally immortalized cell line retain the biological characteristics of intestinal epithelial stem cells and could form intestinal organoids in 3-D cultures. This cell line may be useful for understanding the genetic/epigenetic underpinnings of intestinal tumorigenesis, and/or mouse intestinal crypt biology. In vitro, the cells can be genetically manipulated for further research.
