Genetika člověka
Mikrobiologické aplikace
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Veterinární imunologie
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Intolerance u zvířat
Biochemie
Imunoanalýza
Immortalized Mouse Neuronal Cells (M4b)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Brain |
| Organism |
Mouse (M. musculus) |
| Donor History |
Fetal mouse bred from double heterozygous (Rb 2H/RB 32 Lub) males and normal C57BL females |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions | |
| Immortalization Method |
Supplementation of plating medium with UCHT1 rat thyroid cell line-conditioned medium |
| Full Information |
https://www.abmgood.com/immortalized-mouse-neuronal-cells-m4b.html |
The Immortalized Mouse Neuronal Cells (M4b) were derived from the spinal cord of a fetal mouse after 12-16 days of gestation. The fetal mouse was bred from double heterozygous (Rb 2H/RB 32 Lub) males and normal C57BL females. Transformation of the cell line was induced via supplementation with UCHT1 rat thyroid cell line-conditioned medium and was confirmed via the detection of vimentin. Immunohistochemistry found the presence of neurological makers, but the absence of glial markers in the Immortalized Mouse Neuronal Cells (M4b). In addition, the intracellular [Ca2+] response after neurotransmitter stimulation was rapid and transient as expected. These cells are useful in studies of neuronal cell biology, viral infections, and neuronal cytotoxicity. Additionally, T0674 can be used as a control for the Immortalized Trisomy 16 Mouse Neuronal Cells (MTh) (Cat. No. T0675), which is a mouse model of Down Syndrome.
