Immortalized Mouse Preadipocyte Cells (ScAP-23)

The Immortalized Mouse Preadipocyte Cells (ScAP-23) was isolated from subcutaneous adipose tissue from C57BL/6 mice and immortalized by infection with retroviruses carrying hTERT gene. The ScAP-23 preadipocytes convert to adipocytes when treated with dexamethasone, 3-methylisobutylxanthine, insulin, and indomethacin. Expression of key adipogenic transcription factors such as PPARɣ, C/EBP family, SREBP-1c transcription factors and Krox20/Egr2 are found upregulated during ScAP-23 adipogenesis. The ScAP-23 adipocytes contain abundant lipid droplets and express transcripts for mature adipocytes markers such as SCD1, aFABP, ATGL, FAS, LDL, and GPDH. Furthermore, ScAP-23 adipocytes demonstrate insulin responsiveness and express insulin receptor-β and major insulin-responsive glucose transporter GLUT4. Thus, the cell line is recommended as a tool for adipocytes biology and adipogenesis research.