Immortalized Mouse Renal Proximal Tubule Cells – Conditionally

The renal proximal tubule is part of the nephron duct system in the kidney, playing important roles in functions like absorption and secretion of molecules in the renal system. The Immortalized Mouse Renal Proximal Tubule Cells is a conditionally immortalized mouse renal proximal tubule cells isolated from the mouse (C57BL/6J x Immortomouse, designated as CBA;B10-Tg(H2Kb-tsA58)6Kio/Crl) harboring thermolabile mutation (tsA58) of the simian virus 40 large T antigen. The functional expression of the SV40 large T antigen is induced by culturing the cells in vitro in medium containing IFN γ at a temperature permissive (33°C). At a non-permissive temperature (37°C-39°C), the cells cease to proliferate. These cells retain competent transepithelial electrolyte transport and short-circuit current activities. When paired up with angiotensin receptor (Ang II)-deficient cell lines AT1A-/- (Cat. No. T0626), AT1B-/- (Cat. No. T0627), AT1A-/- AT1B-/- (Cat. No. T0628) and AT2-/- (Cat. No. T0625), these cell lines represent valuable tools to study fluid and electrolyte transportation in the proximal tubule region. RT-PCR was performed to confirm AT receptor genotype.