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Biochemie
Imunoanalýza
Immortalized Porcine Alveolar Macrophage Cells (iPAM303)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Airway |
| Organism |
Pig (Porcine) |
| Donor History |
10-week-old clinically healthy Yorkshire pigs |
| Growth Properties |
Adherent ,fibroblast-like |
| Growth Conditions |
Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow II (TM002) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂ |
| Immortalization Method |
Immortalized with pBABE-SV40LT-Puro and pBABE-hTERT-Puro using PolyMag kit |
| Full Information |
https://www.abmgood.com/immortalized-porcine-alveolar-macrophage-cells-ipam303.html |
PAMs were isolated from ten 10-week-old clinically healthy Yorkshire pigs raised at a local pig farm. Primary porcine alveolar macrophages (PAM) are useful for studying viral infections and immune response in pigs. Immortalized PAM cells possessed the characteristics of primary PAMs including strong expression of swine leukocyte antigen (SLA) class II genes and the inability to grow anchorage-independently. iPAM303 (73.3%+-5.4; SLA DQA*0106/0201; DQB1*0202/0701; DRB1*0402/0602).
