Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
Immortalized Porcine Fibroblast Cell Line (iKUF09)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Ear |
| Organism |
Pig (Porcine) |
| Donor History |
Landrace |
| Growth Properties |
Adherent |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow III (TM003) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method |
Transfection with human telomerase reverse transcriptase (hTERT) and subsequent serial passaging. |
| Full Information |
https://www.abmgood.com/Immortalized-Porcine-Fibroblast-Cell-Line-iKUF09-t0395.html |
The iKUF09 cell line comes from ear tissue of a Landrace pig and exhibits a diverse SLA genotype with significant variation across several alleles, including multiple SLA-1, SLA-2, and SLA-3 alleles. This diversity is critical for studying the genetic and immunological landscape of swine, especially in the context of antigen processing and presentation. The line has been immortalized using hTERT and successfully cultured for over 40 passages, maintaining its fibroblast morphology and rapid growth characteristics.Additional cell lines from this panel:T0390 – Immortalized Porcine Fibroblast Cell Line (iKUF01)T0391 – Immortalized Porcine Fibroblast Cell Line (iKUF02)T0392 – Immortalized Porcine Fibroblast Cell Line (iKUF03)T0393 – Immortalized Porcine Fibroblast Cell Line (iKUF04)T0394 – Immortalized Porcine Fibroblast Cell Line (iKUF07)T0395 – Immortalized Porcine Fibroblast Cell Line (iKUF09)
