Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
Immortalized Rainbow Trout Heart Cells (RTH)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Heart |
| Organism |
Fish |
| Donor History |
Rainbow Trout (O. mykiss) |
| Growth Properties |
Adherent ,cobble-stone |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) coated with Gelatin Coating Solution (0.1%) (TM063) are required for optimal cell adhesion and growth. Prigrow XIII Medium (TM013) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,20.0°C. |
| Immortalization Method |
Immortalized with recombinant retroviruses containing polyoma middle T antigen |
| Full Information |
https://www.abmgood.com/immortalized-rainbow-trout-heart-cells-rth.html |
Immortalized Rainbow Trout Heart Cells (RTH) was generated by transducing rainbow trout heart with recombinant retroviruses carrying polyoma middle T antigen. RTH cells show endothelial-like morphology, are cobblestone in their appearance, and have strong phagocytic activity. Immortalized Rainbow Trout Heart Cells (RTH) constitutively transcribe collagen, selectin, VCAM, several chemokines, and several chemokine receptors. No changes in expression were seen from early passages, up to passage 40. These cells are suitable for study of teleost fish and the role endothelium has in physiological and pathological conditions. It is also suitable for the study of the effect viruses have on endothelial cells due to its susceptibility to VHSV. Evaluation of immune gene expression by real time PCR.
