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Immortalized Rat Conjunctival Epithelial Cells (CJ4.3) – SV40
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Eye |
| Organism |
Rat (R. norvegicus) |
| Donor History |
Fischer 344 Rat |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow IV (TM004) + 0.5% BSA + 10 mM HEPES (TM058) + X trace elements B + 10 μg/ml insulin (TM053) + 5.5 μg/ml transferrin + 50 ng/ml sodium selenate + 20 μg/ml ethanolamine + 0.3 μg/ml glucagon + 0.1 μM dexamethasone + 0.1 μM retinoic acid + 0.3 μM 3 ,3’ ,5 triiodo-L thyronine + 80 ng/ml EGF + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂ |
| Immortalization Method |
Immortalization by transfection with SV40 large T antigen |
| Full Information |
https://www.abmgood.com/immortalized-rat-conjunctival-epithelial-cells-cj4-3-sv40.html |
The Immortalized Rat Conjunctival Epithelial Cells (CJ4.3) were derived from primary conjunctival epithelial cells from Fischer 344 rats. The primary cells were immortalized with pSV(neo) and transfected with SV40 large T antigen. The immortalized cells display epithelial morphology and express the conjunctiva-specific cytokeratin 4, the goblet cell-specific cytokeratin 7 and were negative for the corneal epithelial cell-specific cytokeratin 12. The cells are useful as models for studying the conjunctival epithelial barrier and also in developing strategies for delivering macromolecular antigens across this barrier to underlying components of the immune system. Immunofluorescence confocal microscopy demonstrated cytokeratin expression.
