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Immortalized Rat Embryonic Striatal (M213-2O) Cells – tsSV40T
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Brain |
| Organism |
Rat (R. norvegicus) |
| Donor History |
Sprague-Dawley rat embryo |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow IV (TM004) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,33.0°C ,5% CO₂. |
| Immortalization Method |
Immortalized via infection with retrovirus carrying the A58 temperature-sensitive allele of SV40 large T antigen |
| Full Information |
https://www.abmgood.com/immortalized-rat-embryonic-striatal-m213-2o-cells-tssv40t.html |
Immortalized Rat Embryonic Striatal (M213-2O) Cells – tsSV40T has GABAgeric properties with multiple nucleoli, and no contact inhibition features. It is a tool which can be used to study GABA uptake and release, neural development and differentiation, and is a good model for Huntington’s disease. The cells grown in permissive temperature (33°C) in which SV40 expression is observed. In non-permissive temperature (39.5°C), the morphology of the cells will become elongated and no longer expresses SV40. The growth becomes slower but the cells express GFAP. Has GABAgeric properties with multiple nucleoli, presence of GAD and MAP-2 antigen, absence of vimentin, and no contact inhibition features.
