Genetika člověka
Mikrobiologické aplikace
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Jednokanálové
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Intolerance u zvířat
Biochemie
Imunoanalýza
Immortalized Rat Embryonic Striatal Wildtype (121-1I) Cells
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Brain |
| Organism |
Rat (R. norvegicus) |
| Donor History |
Sprague-Dawley rat embryo ,Straitum |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow IV (TM004) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method |
Immortalized via infection with retrovirus carrying the A58 temperature-sensitive allele of SV40 large T antigen |
| Full Information |
https://www.abmgood.com/immortalized-rat-embryonic-striatal-wildtype-121-1i-cells.html |
Immortalized Rat Embryonic Striatal Wildtype (121-1I) Cells are immortalized with the the A58 temperature-sensitive allele of the SV40 large T antigen. It is a non-GABAergic cell line, in which there is no GAD expression. It may be used as a wild type to compare to the GABAergic cell lines such as Immortalized Rat Embryonic Striatal (M213-2O) Cells – tsSV40T (T0356), Immortalized Rat Embryonic Striatal (M26-1F) Cells – tsSV40T (T0357), or Immortalized Rat Embryonic Striatal (M213-1L) Cells (T0757). A non-GABAergic cell line, in which there is no GAD expression.
