Genetika člověka
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Jednokanálové
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PCR, qPCR, RT
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Reverzní transkripce
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Intolerance
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Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
Immortalized Rat Enteroglial Cells (EGC)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Brain |
| Organism |
Rat (R. norvegicus) |
| Donor History |
Sprague-Dawley Rat ,Male |
| Growth Properties |
Adherent ,stellate shaped |
| Growth Conditions |
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow III (TM003) + 5% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂ |
| Immortalization Method |
Serial passaging and infection by incubating with the supernatant of virus-producing GPE-egfr-neu cell line |
| Full Information |
https://www.abmgood.com/immortalized-rat-enteroglial-cells-egc.html |
Enteroglial cells (EGCs) have a key role in the inflammatory response in the enteric nervous system which is structurally and functionally the largest and most diverse in complexity part of the peripheral nervous system. The Immortalized Rat Enteroglial Cells (EGC) is a valuable tool for studies in the enteric nervous system inflammatory response as it is an immortalized cell line established from the rat myenteric plexus. The cells express low levels of c-fos mRNA but can be up-regulated with stimuli from pro-inflammatory cytokines (IL-1β and TNF-α).Characterized by immunohistochemistry and dot-blot analysis.
