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Immortalized Rat Myoblast-like Tetracycline Cell Line (RMT 6211)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Skeletal Muscle |
| Organism |
Rat (R. norvegicus) |
| Donor History |
6 month old rat |
| Growth Properties |
Adherent ,fibroblast-like |
| Growth Conditions |
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow III (TM003) + 10% FBS + 10% horse serum + 1% Penicillin/Streptomycin Solution (G255) ,33.0°C ,5% CO₂ |
| Immortalization Method |
Immortalized with NIT-Tag retrovirus containing a temperature sensitive SV40 Large T antigen |
| Full Information |
https://www.abmgood.com/immortalized-rat-myoblast-like-tetracycline-cell-line-rmt-6211.html |
Immortalized Rat Myoblast-like Tertracycline Cell Line (RMT 6211) is derived from rat skeletal muscle and have been immortalized via transfection with the NIT-Tag retrovirus, containing a temperature sensitive mutant of the SV40 large T Antigen. This cell line was developed in order to provide a source of myofibers that could exhibit contractile activity similar to that seen in primary muscle cultures. Follwing differentiation, the RMT 6211 cell line was found to express markers for Pax7, MyoD, Myf-5, MRF4, and Myogenin. As a result, this immortalized cell line is a useful alternative to primary cultures and can be used to study muscle differentiation, as well as molecular and physiological aspects of electrical activity in muscle fibres.
