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Immortalized Wild-type Neutrophil Progenitor Cells (mSB8e-C57)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Bone Marrow |
| Organism |
Mouse (M. musculus) |
| Donor History | |
| Growth Properties |
Suspension |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) are recommended for optimal cell culture; passage to new plastic with fresh media at least every 4 days. PriGrow II (TM002) + 10% FBS + 100 ng/ml Stem Cell Factor (SCF) + 0.5 μM ß-estradiol (0.5 μM) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. ß-estradiol is required for cell maintenance but not differentiation. Note: cells are sensitive to freeze thaw conditions. Do not thaw cells completely; immediately spin down cells and re-suspend in complete growth medium supplemented with 15% FBS to help cells recover for the first 24 hours. For information regarding cell differentiation ,please refer to the Differentiation Protocol PDF under the Documents Tab. |
| Immortalization Method |
Serial passaging and transduction with retrovirus carrying Hoxb8 (MSCVneo-HA-ER-Hoxb8) |
| Full Information |
https://www.abmgood.com/Immortalized-Wild-type-Neutrophil-Progenitor-Cells-mSB8e-C57-t0209.html |
This cell line (Cat. T0209) is the parental, wild-type form of the immortalized neutrophil progenitor cells used to create Cat. T0202 and Cat. T0204. These cells are a useful model for immunological reasearch. Additional cell lines within this panel include:Cat. T0200 – Immortalized Myeloid Granulocyte Cell Line (ECoM-G)Cat. T0201 – Immortalized Myeloid Monocyte Cell Line (ECoM-M)Cat. T0202 – Immortalized Myeloid Neutrophil Cells (mSB8e-C57STEM-ERKO)Cat. T0203 – Immortalized Myeloid Monocyte Cells (mGB8e-C57STEM-ERKO)Cat. T0204 – Immortalized Neutrophil Cells (mSB8e-C57-Cas9GFP)Cat. T0205 – Immortalized Myeloid Monocyte Cells (mGB8e-C57-Cas9GFP)Cat. T0207 – Immortalized Wild-type Monocyte Progenitor Cells (mGB8e-C57)Cat. T0209 – Immortalized Wild-type Neutrophil Progenitor Cells (mSB8e-C57)
