KYSE-410 Cells

KYSE-410 is a human esophageal squamous cell carcinoma (ESCC) cell line that was established from a primary tumor resected from an adult patient. This cell line is part of the KYSE series, which includes multiple ESCC models designed to provide a comprehensive tool for studying the various aspects of esophageal cancer. KYSE-410 cells have a doubling time of 24.2 hours, reflecting a moderate proliferative capacity. They grow as adherent monolayers, a common feature among epithelial-derived cancer cells, and exhibit a relatively uniform morphology under phase-contrast microscopy.
At the genetic level, KYSE-410 is particularly notable for its epigenetic alterations. The p16 (INK4a) gene in KYSE-410 shows hypermethylation of the 5′ CpG islands, a modification that leads to the silencing of this crucial tumor suppressor gene. This epigenetic change is a significant driver of oncogenesis in many cancers, including ESCC, as it results in the loss of cell cycle regulation and unchecked cell proliferation. Despite this, KYSE-410 retains a wild-type configuration for the p15 (INK4b) gene, highlighting a selective inactivation of p16 that is typical of certain cancer subtypes.
The KYSE-410 cell line is tumorigenic, as demonstrated by its ability to induce tumor formation when implanted into athymic nude mice. The histological analysis of these tumors shows features consistent with squamous cell carcinoma, making KYSE-410 a relevant model for in vivo studies. This cell line is highly valuable for research focused on understanding the role of epigenetic modifications in cancer progression, as well as for testing the efficacy of therapies targeting epigenetic regulators, although it is not intended for therapeutic or in vivo applications.