L5178-R Cells

Unit

5 x 10^6 cells/ml

Cell Type

Tumor Cells

Tissue Type

Mouse cell lines

Organism

Mouse

Donor History

Leukemia

Growth Properties

Suspension

Growth Conditions

Culture medium: RPMI 1640

,

w: 2.0 mM stable Glutamine

,

w: 2.0 g/L NaHCO3 (Cytion article number 820700a). Supplements: Supplement the medium with 10% FBS

,

1 mM sodium pyruvate

,

1% NEAA. Seeding density: 1 x 10⁶ cells/ml. Freeze medium: As a cryopreservation medium

,

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

,

or CM-1 (Cytion catalog number 800100)

,

which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

,

5% CO2

,

humidified atmosphere.

Immortalization Method
Full Information

https://www.cytion.com/L5178-R-Cells/400258

SKU: 400258 Kategorie: ,
Mgr. Otakar Fišer
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Mgr. Otakar Fišer
Produktový manažer - Life Science
Popis

The L5178-R cell line is a murine lymphoma cell line derived from mouse lymphoid tissues. This cell line is particularly notable for its use in studying the mechanisms of lymphomagenesis and the cellular responses to various treatments, including chemotherapeutic agents and radiation. L5178-R cells are radioresistant, which makes them a valuable model for exploring the molecular and genetic factors that contribute to radiation resistance in cancer cells. This attribute is essential for research into improving therapeutic strategies for treating resistant forms of cancer.

L5178-R cells are also frequently employed in mutagenesis and carcinogenesis studies due to their high sensitivity to mutagenic agents. This sensitivity is exploited in assays that assess the mutagenic potential of chemical compounds, contributing to toxicological research and safety evaluations. The cell line’s genetic and phenotypic characteristics provide a robust platform for in vitro studies, enabling scientists to dissect the pathways involved in cancer development and progression. Additionally, the L5178-R cell line is used in immunological research to understand the interaction between tumor cells and the immune system, aiding in the development of immunotherapeutic approaches.

O značce

Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.

Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.