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Imunoanalýza
LGD 1069 Resistant A375 Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Skin |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,54 ,Caucasian ,Amelanotic melanoma |
| Growth Properties |
Adherent ,Epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 2% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method |
Tumor Cell Line |
| Full Information |
https://www.abmgood.com/lgd-1069-resistant-a375-cell-line.html |
The human melanoma cell line, A375, was grown in low dose of 10 nM LGD 1069, pioglitazone (a thiazolidine), and a combination of the two (50% each). Concentrations were increased every 1-2 weeks until cells were growing at a concetration of 100 n M – previously inhibitory to cell growth. Cells were treating for approximately 14 weeks. Cell line authentication was confimed through STR profiling and dexoyribonucleic acid profiliing analysis. These cells are usedul for studying disease mechanisms and for developing targeted drug therapies.
