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Lhx5 Stable Neuro2 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Brain |
| Organism |
Mouse (M. musculus) |
| Donor History |
Neuroblastoma |
| Growth Properties |
Adherent ,neuronal |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 1.0 mg/ml Geneticin/G418 (G271) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
Lhx5 is a transcription factor with restricted Mamillary body (MBO) expression. LHX5 is a member of the LHX family of transcription factors acting as important differentiation determinants, and it is strongly expressed in the caudal hypothalamus from very early stages (E9.5) and plays a specific role in forebrain development. Lhx5 has an essential role in several different developmental pathways regulating MBO specification and differentiation. This cell line can be used to study the mechanisms behind how neuronal identity are integrated with upstream regional specification networks.
