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Imunoanalýza
Luciferase (Luc2) Expressing TRAMP-C1 Stable Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Prostate |
| Organism |
Mouse (M. musculus) |
| Donor History |
Carcinoma of the mouse prostate gland |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 2.5 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/luciferase-luc2-stable-tramp-cell-line.html |
Luciferase is commonly used as a reporter to assess the transcriptional activity of a genetic construct with luciferase gene under the promoter of interest. Luciferase stable TRAMP Cells is a luciferase expressing cell line derived from TRAMP cells. The expression of luciferase has been confirmed with luciferase assay. All clones are puromycin (2.5 μg/ml)-resistant. Luciferase stable TRAMP cells can be a very useful cell line for non-invasive visualization in animal models.
