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Luciferase Stable 17L3C (17L3C-luc) Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Mammary |
| Organism |
Mouse (M. musculus) |
| Donor History |
Syngeneic FVB mammary tumor |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 50 µg/ml Geneticin/G418 (G271) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/luciferase-stable-17l3c-17l3c-luc-cell-line.html |
Luciferase is a class of oxidative enzymes that produce bioluminescence when expressed in cells they are used for microscopy and as a reporter gene. 17L3C cells were derived from a mammary tumor in a female MMTV-PyVT transgenic mouse (FVB/n strain). Luciferase Stable 17L3C (17L3C-luc) Cell Line presents a model which allows real-time observations of tumor growth in vivo.
