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Imunoanalýza
MAPRE1 Doxycycline Inducible Knockout HeLa Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Cervix |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,31 ,Cervical carcinoma |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/mapre1-doxycycline-inducible-knockout-hela-cell-line.html |
This human cell line stably expresses single guide RNA (sgRNA) targeting an early exon of MAPRE1 (microtubule-associated protein RP/EB family member 1). Cas9 expression is induced by the presence of doxycycline enabling inducible knockout of the gene of interest. A PiggyBac transposon system was used to introduce Cas9 while guide RNAs were introduced using lentiviral infection.
