MARC-145 Cells

Unit

3 x 10^6 cells/ml

Cell Type

Immortalized Cells

Tissue Type

Monkey cell lines

Organism

Chlorocebus pygerythrus (Vervet monkey)

Donor History
Growth Properties

Adherent

Growth Conditions

Culture medium: DMEM

,

w: 4.5 g/L Glucose

,

w: 4 mM L-Glutamine

,

w: 3.7 g/L NaHCO3

,

w: 1.0 mM Sodium pyruvate (Cytion article number 820300a). Supplements: Supplement the medium with 10% FBS. Dissociation reagent: Accutase. Freeze medium: As a cryopreservation medium

,

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

,

or CM-1 (Cytion catalog number 800100)

,

which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

,

5% CO2

,

humidified atmosphere.

Immortalization Method
Full Information

https://www.cytion.com/MARC-145-Cells/305006

SKU: 305006 Kategorie: ,
Mgr. Otakar Fišer
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Mgr. Otakar Fišer
Produktový manažer - Life Science
Popis

The MARC-145 cell line is a monkey kidney epithelial cell line derived from the African green monkey (Cercopithecus aethiops). This cell line is particularly notable for its use in virology, especially in the propagation of porcine reproductive and respiratory syndrome virus (PRRSV), a significant pathogen in the swine industry. MARC-145 cells have been instrumental in studying PRRSV due to their high susceptibility to the virus, making them a valuable tool for viral isolation, propagation, and vaccine development.
MARC-145 cells are characterized by their epithelial morphology and robust growth in vitro, which facilitates large-scale production of PRRSV for research purposes. Additionally, they have been used to explore the mechanisms of PRRSV infection, including virus entry, replication, and host-pathogen interactions. This cell line has also been utilized in the development and testing of antiviral compounds and PRRSV vaccines, contributing significantly to efforts aimed at controlling this economically important disease in pigs.

O značce

Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.

Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.