MIN-6 Cells
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Mouse cell lines |
| Organism |
Mouse |
| Donor History |
Mouse insulinoma |
| Growth Properties |
Adherent |
| Growth Conditions |
Culture medium: DMEM ,w: 4.5 g/L Glucose ,w: 4 mM L-Glutamine ,w: 3.7 g/L NaHCO3 ,w: 1.0 mM Sodium pyruvate (Cytion article number 820300a). Supplements: Supplement the medium with 15% heat-inactivated FBS ,50 µM beta-Mercaptoethanol. Dissociation reagent: Accutase. Seeding density: 5 x 10⁴ cells/cm². Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
The MIN-6 cell line is a murine pancreatic beta cell line derived from insulinoma. It is commonly used in research to study insulin secretion mechanisms and beta-cell function due to its ability to synthesize and secrete insulin in response to glucose levels. This cell line is particularly valuable because it retains many of the functional characteristics of primary pancreatic beta cells, making it a useful model for diabetes research.
MIN-6 cells exhibit glucose-responsive insulin secretion, which is a critical trait for studies focusing on the regulation of insulin release and the cellular responses to varying glucose concentrations. The cells are also used to investigate pancreatic beta-cell proliferation and apoptosis, as well as the role of various genes and environmental factors in these processes. Additionally, MIN-6 cells have been instrumental in testing potential pharmacological agents for their effects on beta-cell function and survival, thus contributing to the development of new therapeutic strategies for diabetes.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Třepačky
Suché lázně
Fluorometry
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Čtečky identifikačních čipů
Termočipy
Biochemie
Imunoanalýza

