Genetika člověka
Mikrobiologické aplikace
NGS
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PCR plasty
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Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
MRGPRX4 Expressing Stable 293T Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Human (H. sapiens) |
| Donor History |
Embryo |
| Growth Properties |
Adherent |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 100 µg/ml Hygromycin (G265) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/MRGPRX4-Expressing-Stable-293T-Cell-Line.html |
This cell line stably express the human Mas-related G protein-coupled receptor X4 (MRGPRX4) in 293T cells. These cells are suitable for in-depth analysis of MRGPRX4, crucial for understanding its role in modulating immune and sensory pathways. This cell line offers a stable expression system for performing reproducible β-arrestin recruitment and calcium mobilization assays, facilitating the exploration of receptor dynamics and ligand interactions. It is optimized for high-throughput applications – providing a vital resource for the identification of novel therapeutic targets and the advancement of drug discovery in the realm of GPCR-related diseases and conditions.Additional cell lines form this panel: Cat. T3215 – MRGPRX2 Expressing Stable 293T Cell Line
