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Mutant AKT1 Stable Proteus Syndrome Derived Cell Line (F6B)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Skin |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,4-8 years ,Asian ,Epidermal nevus on hand |
| Growth Properties |
Adherent; Fibroblast ,elongated flattened cells |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/mutant-akt1-stable-proteus-syndrome-derived-cell-line-f6b.html |
This cell line is derived from tissue obtained from Proteus syndrome patients. These cells contain only the E17K AKT1 mutation, making them suitable for studying the effects of a single deviation on cell growth and metabolism. Furthermore, this cell line is suitable for proteus syndrome drug therapy development. AKT1 expression was detected through PCR based RFLP assay genotyping. When grown in serum-free medium, cells have increased pAKT levels compared to wild-type cells.
