NCI-N87 Cells

Unit

3 x 10^6 cells/ml

Cell Type

Tumor Cells

Tissue Type

Gastric cancer cell lines

,

NCI cell lines

Organism

Human

Donor History

Gastric tubular adenocarcinoma

Growth Properties

Adherent

Growth Conditions

Culture medium: RPMI 1640

,

w: 2.0 mM stable Glutamine

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w: 2.0 g/L NaHCO3 (Cytion article number 820700a). Supplements: Supplement the medium with 10% FBS

,

10 mM HEPES

,

2

,

5g/L Glucose and 1mM Sodiumpyruvate. Dissociation reagent: Accutase. Freeze medium: As a cryopreservation medium

,

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

,

or CM-1 (Cytion catalog number 800100)

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which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

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5% CO2

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humidified atmosphere.

Immortalization Method
Full Information

https://www.cytion.com/NCI-N87-Cells/305057

SKU: 305057 Kategorie: ,
Mgr. Otakar Fišer
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Mgr. Otakar Fišer
Produktový manažer - Life Science
Popis

NCI-N87, also known as N87, is a human gastric cancer cell line and is widely utilized in cancer research, particularly gastric carcinoma studies.
NCI-N87 cells contribute to our understanding of the digestion model of the gastric mucosa and play a role in the development of gastroretentive delivery systems. In pharmacological contexts, NCI-N87 cells have been used to explore the role of gentamicin as an anticancer agent.
The gastric adenocarcinoma cell line NCI-N87 is tumorigenic and expresses the oncogenes myc and erb-B2, and are therefore instrumental in xenograft model studies. This cell line’s inflammatory properties and response to agents like gentamicin can be assayed, as can its potential involvement in epithelial barrier integrity and function using intestinal permeability assays.
The cells are known to express surface glycoproteins such as carcinoembryonic antigen (CEA) and TAG 72, but are negative for L-dopa decarboxylase (DDC). The cells show minimal positivity for vasoactive intestinal peptide (VIP) receptors and lack gastrin receptors, and they express receptors for muscarinic cholinergic agents. No amplification or rearrangements were observed in N-myc, L-myc, myb, and EGF receptor genes in these cells.
In summary, the gastric epithelium cell line NCI-N87 serves as a model for gastric cancer research, epithelial cell behavior, drug delivery systems, and the metabolic pathways of nutritionally relevant compounds.

O značce

Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.

Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.