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NFAT‐luc‐CMVH Stably Expressing RAW264.7 Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Ascites |
| Organism |
Mouse (M. musculus) |
| Donor History |
Male ,Balb/c ,Adult ,Abelson murine leukemia virus-induced tumor |
| Growth Properties |
Adherent ,dendritic-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. MEM α ,No Nucleosides Medium (TM512) + 10% FBS + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
The NFAT-luc-CMVH Stable RAW264.7 Cell Line is a genetically engineered version of the RAW264.7 murine macrophage cell line, which stably expresses a luciferase reporter gene under the control of the Nuclear Factor of Activated T-cells (NFAT) promoter, combined with a cytomegalovirus (CMV) enhancer. This cell line is an essential tool for investigating the activation of NFAT, a key transcription factor involved in the regulation of immune response and in the development of T cells. The luciferase reporter allows for the real-time, non-destructive quantification of NFAT activation by emitting bioluminescence in response to calcium signaling, making it highly suitable for drug screening and cellular communication studies. This cell line is particularly useful in studies focused on understanding the dynamics of macrophage activation and the cellular mechanisms underlying inflammatory responses. Additionally, it can be used to assess the impact of pharmacological agents on NFAT pathway activation within macrophages, providing insights into potential therapeutic targets for diseases involving immune dysregulation.Complete list of cell lines from this panel:Cat. T6529 – NFAT‐luc‐CMVH Stably Expressing RAW264.7 Cell LineCat. T6530 – AP‐1‐luc‐CMVH Stably Expressing RAW264.7 Cell LineCat. T6531 – ARE‐luc‐CMVH Stably Expressing RAW264.7 Cell Line
