Normal Human Primary Hepatic Kupffer Cells

Unit

1×10⁶ cells / 1.0 ml

Cell Type

Primary Cells

Tissue Type

Liver

Organism

Human (H. sapiens)

Donor History

Normal liver

Growth Properties

Adherent

,

polygonal

Growth Conditions

PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM5549) + 1% Penicillin/Streptomycin Solution (G255)

,

37.0°C

,

5% CO₂.Note: Cells require additional time to recover from post-thaw conditions. Change media every day after the first 48 hours post-thaw

,

or earlier if media starts to change colour to yellow. Cells are terminally differentiated and can be maintained in culture for up to 7 days. 

Markers

D68

,

TNF-a

,

IL-6

,

CD34

,

F4/80 Antibody

Full Information

https://www.abmgood.com/normal-human-primary-hepatic-kupffer-cells.html

Katalogové číslo: T5549 Kategorie: ,
Popis

Kupffer Cells, also known as Browicz-Kupffer cells and stellate macrophages, are specialized macrophages located in the liver lining the walls of the sinusoids. Upon activation Kupffer cells release various products like cytokines, prostanoides, nitric oxide and reactive oxygen species. These factors determine the phenotypes of the Kupffer Cells and their neighbouring cells, such as hepatocytes, stellate cells, endothelial cells and other immune cells that travel through the liver. Therefore, kupffer cells are perfect for studying liver’s response to infection, toxins, ischemia, resection and other stresses.

O značce
Applied Biological Materials (abm) je jedním z našich klíčových dodavatelů pro biotechnologický průmysl. Společnost byla založena v roce 2004 a již více než deset let podporuje vědecké objevy v oblasti přírodních věd a vývoje léčiv. Její sídlo se nachází ve Vancouveru v Kanadě. ABM je pro nás neocenitelným partnerem zejména v  oblasti dodávek buněčných kultur, genových a virových vektorů, produktů pro molekulární biologii a PCR, nástrojů pro technologie CRISPR a RNAi, protilátek i základních reagencií pro molekulárně genetické procesy.