Normal Human Primary Hepatic Kupffer Cells
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Primary Cells |
| Tissue Type |
Liver |
| Organism |
Human (H. sapiens) |
| Donor History |
Normal liver |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions |
PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM5549) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂.Note: Cells require additional time to recover from post-thaw conditions. Change media every day after the first 48 hours post-thaw ,or earlier if media starts to change colour to yellow. Cells are terminally differentiated and can be maintained in culture for up to 7 days. |
| Markers |
D68 ,TNF-a ,IL-6 ,CD34 ,F4/80 Antibody |
| Full Information |
https://www.abmgood.com/normal-human-primary-hepatic-kupffer-cells.html |
Kupffer Cells, also known as Browicz-Kupffer cells and stellate macrophages, are specialized macrophages located in the liver lining the walls of the sinusoids. Upon activation Kupffer cells release various products like cytokines, prostanoides, nitric oxide and reactive oxygen species. These factors determine the phenotypes of the Kupffer Cells and their neighbouring cells, such as hepatocytes, stellate cells, endothelial cells and other immune cells that travel through the liver. Therefore, kupffer cells are perfect for studying liver’s response to infection, toxins, ischemia, resection and other stresses.
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Třepačky
Suché lázně
Fluorometry
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
