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Imunoanalýza
osbpl2 CRISPR Knock Out NIH/3T3 Cell Line – T1 Polyclonal Pool
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Embryo |
| Organism |
Mouse (M. musculus) |
| Donor History |
Male ,NIH Swiss ,Embryo |
| Growth Properties |
Adherent ,fibroblast |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 0.6 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/osbpl2-crispr-knock-out-nih-3t3-cell-line-t1-polyclonal-pool.html |
Derived from mouse embryo, this NIH/3T3 cell line features a CRISPR knockout of the Oxysterol Binding Protein Like 2 (osbpl2). This cell line is well suited for DNA transfection.
