PARP-1 Knockout Immortalized Mouse Heart Endothelial (HYKO6) Cell Line

Poly(ADP-ribose) polymerase 1 (PARP-1) is an ADP-ribosylating enzyme that plays a critical role in DNA damage repair, in addition to functioning as a context- specific regulator of transcription factors. Various pathophysiological conditions, such as reperfusion injury, endotoxic shock, diabetes and aging are associated with endothelial cell dysfunction mediated by PARP-1. <br></br>The PARP-1^-/-Knockout Immortalized Mouse Heart Endothelial (HYKO6) Cell Line is derived from stable transformation of SV40 into primary 9-10 week old 129/Sv x C57BL/6 mouse heart endothelial cells deficient for PARP-1. This cell line is capable of morphologic differentiation in capillary-like structures (CLS), similar to its primary counterparts, as shown by branching and anastomosing cords of cell networks and tube formation after 12 hours culturing on Matrigel. Moreover, HYKO6 responds to cytokine stimulation and has been shown to exhibit increased VCAM-1 and E-selectin expression after TNFα stimulation, making this cell line an important tool in the elucidation of PARP-1 signaling pathway involved in endothelial cell dysfunction and molecular mechanisms controlling cell gene expression in response to inflammatory stimuli.