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PIK3CA (H1047R) Mutant Mammary Stable Cell Line (W.3tg.HR2)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Mammary |
| Organism |
Mouse (M. musculus) |
| Donor History |
Female ,C57Bl/6 and FVB/N |
| Growth Properties |
Adherent ,mesenchymal |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 15 mM HEPES (TM058) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
This murine cell lines features a mutant form of PIK3CA H1047R isolated from a tumor developed in a triple transgenic mice PIK3CA.WAP-Cre.lsl-PBase. The PIK3CA H1047R mutation is known to play a significant role in the development and progression of various cancers, including breast cancer. Cell lines harboring this mutation, such as the W.3tg.HR2, are critical for studying the molecular mechanisms underlying cancer proliferation, survival, and metastasis, alongside the development of new cancer therapeutics.
