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PINK1(F104M)-YFP-IRES-DsRed Expressing HCT116 Stable Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Colon |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,58 ,Caucasian ,Colorectal carcinoma |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. McCoy's 5A Medium (TM506) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
The PINK1(F104M)-YFP-IRES-DsRed Expressing HCT116 Stable Cell Line (T3511) expresses a mutant form of PTEN-induced putative kinase 1 (PINK1) with a phenylalanine to methionine substitution at position 104 (F104M), tagged with Yellow Fluorescent Protein (YFP) and linked to DsRed through an Internal Ribosome Entry Site (IRES). This mutation enhances the stability of PINK1 in the cytosol, allowing for in-depth study of its function and interaction with mitochondrial components. The cell line is crucial for investigating the effects of PINK1 stabilization on mitochondrial quality control, autophagy, and its potential implications in Parkinson’s disease, particularly in understanding how mutations affect PINK1’s role in mitochondrial dynamics and mitophagy.Additional cell lines from this panel:Cat. T3510 – PINK1-YFP-IRES-DsRed Expressing HCT116 Stable Cell Line
