Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
PPOX-Trp227 Stable Knockout U-2 OS Cell Line (PPOXTrp227: W227/1)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Bone |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,15 ,Osteosarcoma |
| Growth Properties |
Adherent ,fibroblast |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/PPOX-Trp227-Stable-Knockout-U-2-OS-Cell-Line-PPOXTrp227:-W227/1-T3479.html |
Featuring a CRISPR/Cas9-induced knockout at the Trp227 site of the PPOX gene, this cell line is devoid of PPOX activity, leading to pronounced accumulation of protoporphyrinogen IX and a halving of heme levels compared to controls. Despite these biochemical alterations, cell proliferation remains unaffected, highlighting the cell’s adaptive mechanisms to heme deficiency. This model is indispensable for studying the pathophysiology of heme-related disorders and testing novel therapeutic agents targeting the heme biosynthetic pathway.Complete list of cell lines from this panel:Cat. T3477 – PPOX-Cys459 Stable Knockout U-2 OS Cell Line (PPOXCys459: C459/1)Cat. T3478 – PPOX-Arg38 Stable Knockout U-2 OS Cell Line (PPOXArg38: R38/1)Cat. T3479 – PPOX-Trp227 Stable Knockout U-2 OS Cell Line (PPOXTrp227: W227/1)
