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Biochemie
Imunoanalýza
PROL1 Knockout Prostate Cancer Cell Line (LNCaP-PROL1-)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Prostate |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,Caucasian ,50 ,Prostate carcinoma |
| Growth Properties |
Adherent ,Epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 1.5 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/PROL1-Knockout-Prostate-Cancer-Cell-Line-LNCaP-PROL1-t3240.html |
PROL1 overexpresison has been associated with cancer, playing a role in overcoming the hypoxic barrier that develops as tumors grow. LNCaP cells were transduced using a commercially available PROL1 CRISPR knockout kit according to manufacturers’ protocol. Following selection with with 5 Pg/ml puromycin, it was confirmed that each colony was expressing GFP before pooling colonies and confirming knock- out of PROL1 across the non-clonal population. RNAseq and qt-RT- PCR was used to confirm knockout of PROL1. In the LNCaP-ProL1- cell line PROL1 was below the limit of detection by qt-RT-PCR.
