PY8119-GFP

PY8119-GFP cells are a fluorescently labeled derivative of the murine PY8119 breast cancer cell line, which originates from a spontaneous mammary tumor in a mouse model. The parental PY8119 line is commonly associated with genetically engineered mouse models of breast cancer and is used to study tumor progression, immune interactions, and therapeutic responses in a syngeneic context. Stable expression of green fluorescent protein (GFP) enables direct visualization of tumor cell behavior, facilitating real-time tracking of proliferation, migration, and cellular interactions both in vitro and in vivo.

The GFP modification makes PY8119-GFP cells particularly well suited for applications such as live-cell imaging, intravital microscopy, and metastasis studies in immunocompetent mouse models. These cells retain key characteristics of the parental line, including aggressive growth and the ability to form tumors when implanted into syngeneic hosts. As a result, PY8119-GFP cells are widely used to investigate tumor–immune system dynamics, evaluate anti-cancer therapies, and study mechanisms of invasion and dissemination. However, as with all fluorescently modified lines, careful validation is recommended to ensure that GFP expression does not alter baseline cellular behavior.

Genetic modification: Stably modified by replication-incompetent lentiviral transduction to express the ZsGreen1 green fluorescent protein reporter; maintained as a polyclonal population under puromycin selection (1–5 µg/mL). S1/BSL-1 containment.