R2 Mutant HepG2 Stable Cell Line (R2-mut)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Liver |
| Organism |
Human (H. sapiens) |
| Donor History |
Hepatoma |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 8% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/R2-Mutant-HepG2-Stable-Cell-Line-R2-mut-t3316.html |
This cell line features a mutant in the R2-box (a region within the ERE that is highly homologous to the R2 5′UTR) region of the R2 gene. Cells express very low levels of R2 transcript and protein resulting in slower growth compared to wild-type HepG2 cells. Since R2 regulates the HBV cell cycle, this cell line is suitable for studies regarding HBV infectin and pathogensis.Note: Keep cells at low passage.
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Třepačky
Suché lázně
Fluorometry
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
