Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
Rieske ISP Stable Knockout Mouse Fibroblast Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Lung |
| Organism |
Mouse (M. musculus) |
| Donor History | |
| Growth Properties |
Adherent ,fibroblast |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1 mM Sodium Pyruvate (TM057) + 50 µg/ml Uridine + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/rieske-isp-stable-knockout-mouse-fibroblast-cell-line.html |
To generate the Rieske ISP Stable Knockout Mouse Fibroblast Cell Line; primary lung fibroblast culture was established from a knockin mouse homozygous for the floxed UQCRFS1 (exon 2) gene. UQCRFS1 encodes for the Rieske iron sulfur protein subunit of complex III. Cells were immortalized by retroviral transduction with E6/E7 type 16 papiloma virus carrying G418 resistance. Clones were obtained from immortalization. One of the clones #8 was used to generate the RIESKE KO cells by transfecting with a plasmid expressing cre recombinase. Clones were obtained after hygromycin selection.
