Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
Ryanodine Receptor 1 Stable HEK293 Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Human (H. sapiens) |
| Donor History |
Embryo |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 15% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 100 µg/ml Hygromycin (G265) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/ryanodine-receptor-1-stable-hek293-cell-line.html |
Ryanodine receptors (RyR) are intracellular calcium channels located in the sarcoplasmic reticulum and the endoplasmic reticulum, and they mediate the release of calcium ions during muscle contractions. RyR are structurally and functionally similar to the inositol 1,4,5-triphosphate receptors (IP3R), which is responsible in initiating intracellular calcium signaling pathways. Mutations to the receptor have been observed in numerous diseases states. The Ryanodine Receptor 1 Stable HEK293 Cell Line stably expresses functional rabbit RyR1 calcium release channels, thus providing a valuable tool for pharmacological investigations in the function and mechanisms of calcium release channels activity, and in depth biophysical studies of the RyR1 receptor. Western blot and immunofluorescence analysis was used to confirm the expression of rabbit RyR1.
