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SCCF1 Stably Expressing Luciferase Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Larynx |
| Organism |
Cat (Feline) |
| Donor History |
Cat ,Laryngeal squamous cell carcinoma |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow III (TM003) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂.0.4 mg/ml Geneticin/G418 (G271) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/sccf1-stably-expressing-luciferase-cell-line.html |
Developed from a laryngeal squamous cell carcinoma of a cat. The cells were stably transfected with a plasmid containing a luciferase-YFP fusion construct. SCCF1 cells can be used for experiments on genetic dysregulation in neoplastic keratinocytes of the feline oropharynx. Will form tumors in nude mice, but do not readily invade bone. These cells are a useful in vitro model for head and neck cancer.
