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Scrambled (spCas9) Negative Control MPNST JW23.3 Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Nerve |
| Organism |
Mouse (M. musculus) |
| Donor History |
Male ,104-day ,C57BL6/J Nf1±;Trp53±cis (NPcis) mice ,Jaw tumor |
| Growth Properties |
Adherent |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 1.0 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
The malignant peripheral nerve sheath tumor (MPNST) lines were established from male C57BL6/J mice with specific genetic mutations inherited from the father: Nf1+/− (heterozygous for the NF1 gene) and Trp53+/− (heterozygous for the p53 gene), in a cis configuration. Cells from jaw tumor of a 104-day old mouse were designated as JW23 cells. This cell line stably expresses spCas9.
