SK-CO-1 Cells

Unit

3 x 10^6 cells/ml

Cell Type

Tumor Cells

Tissue Type

Gastric cancer cell lines

,

Human cells

Organism

Human

Donor History

Colorectal Adenocarcinoma

Growth Properties

Adherent

Growth Conditions

Culture medium: EMEM (MEM Eagle)

,

w: 2 mM L-Glutamine

,

w: 2.2 g/L NaHCO3

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w: EBSS (Cytion article number 820100a). Supplements: Supplement the medium with 10% FBS and 1% NEAA. Dissociation reagent: Accutase. Freeze medium: As a cryopreservation medium

,

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

,

or CM-1 (Cytion catalog number 800100)

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which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

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5% CO2

,

humidified atmosphere.

Immortalization Method
Full Information

https://www.cytion.com/SK-CO-1-Cells/305626

SKU: 305626 Kategorie: ,
Mgr. Otakar Fišer
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Mgr. Otakar Fišer
Produktový manažer - Life Science
Popis

The SK-CO-1 cell line is a human colorectal adenocarcinoma model derived from a metastatic site in ascitic fluid. It has been widely used in cancer research to study the molecular mechanisms underlying colorectal cancer (CRC) progression and response to therapeutic interventions. SK-CO-1 cells are adherent in culture and exhibit morphological characteristics consistent with epithelial tumor cells. This cell line has been included in large-scale genomic studies, such as the Cancer Cell Line Encyclopedia (CCLE), which provides comprehensive genetic, transcriptomic, and pharmacological profiling.

Genetic studies on SK-CO-1 have identified mutations and copy number variations in genes critical to CRC pathogenesis, including alterations in TP53, KRAS, and APC. These features make it a valuable model for exploring pathways such as WNT/β-catenin signaling, which plays a significant role in colorectal tumor development. Furthermore, pharmacological screening has revealed the cell line’s differential sensitivities to chemotherapeutic agents, helping researchers identify potential biomarkers for drug response.

O značce

Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.

Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.