SK-CO-1 Cells
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Gastric cancer cell lines ,Human cells |
| Organism |
Human |
| Donor History |
Colorectal Adenocarcinoma |
| Growth Properties |
Adherent |
| Growth Conditions |
Culture medium: EMEM (MEM Eagle) ,w: 2 mM L-Glutamine ,w: 2.2 g/L NaHCO3 ,w: EBSS (Cytion article number 820100a). Supplements: Supplement the medium with 10% FBS and 1% NEAA. Dissociation reagent: Accutase. Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
The SK-CO-1 cell line is a human colorectal adenocarcinoma model derived from a metastatic site in ascitic fluid. It has been widely used in cancer research to study the molecular mechanisms underlying colorectal cancer (CRC) progression and response to therapeutic interventions. SK-CO-1 cells are adherent in culture and exhibit morphological characteristics consistent with epithelial tumor cells. This cell line has been included in large-scale genomic studies, such as the Cancer Cell Line Encyclopedia (CCLE), which provides comprehensive genetic, transcriptomic, and pharmacological profiling.
Genetic studies on SK-CO-1 have identified mutations and copy number variations in genes critical to CRC pathogenesis, including alterations in TP53, KRAS, and APC. These features make it a valuable model for exploring pathways such as WNT/β-catenin signaling, which plays a significant role in colorectal tumor development. Furthermore, pharmacological screening has revealed the cell line’s differential sensitivities to chemotherapeutic agents, helping researchers identify potential biomarkers for drug response.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Třepačky
Suché lázně
Fluorometry
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Čtečky identifikačních čipů
Termočipy
Biochemie
Imunoanalýza

