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Biochemie
Imunoanalýza
Stable Bcl-x Knockout Mouse Embryonic Fibroblast Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Embryo |
| Organism |
Mouse (M. musculus) |
| Donor History | |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/stable-bcl-x-knockout-mouse-embryonic-fibroblast-cell-line.html |
The Stable Bcl-x Knockout Mouse Embryonic Fibroblast Cell Line was generated from embryos of a pregnant mouse heterozygous for the bcl-x gene (bcl-x+/−). This stable cell line lacks the ability to express B-cell lymphoma-extra-large (Bcl-xL) but this deficiency did not induce compensatory changes in the expression of other Bcl-2 proteins. The Stable Bcl-x Knockout Mouse Embryonic Fibroblast Cell Line showed increased sensitivity to various apoptotic stimuli. This cell line is also capable of re-expressing wild-type and Bcl-xL mutants which makes it a useful research tool in demonstrating how apoptotic regulation is involved in disease development.
