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Imunoanalýza
Stable Bcl-xL Mouse Embryonic Fibroblast (ER) Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Embryo |
| Organism |
Mouse (M. musculus) |
| Donor History | |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/stable-bcl-xl-mouse-embryonic-fibroblast-er-cell-line.html |
The Stable Bcl-xL Mouse Embryonic Fibroblast (ER) Cell Line was derived from embryos of a pregnant mouse heterozygous for the bcl-x gene (bcl-x +/-). After immortalization by transfection of the SV40 large T antigen, the mouse embryonic fibroblasts were transfected with mutant Bcl-xL cDNA. This stable cell line is able to express B-cell lymphoma-extra-large (Bcl-xL) localized in the endoplasmic reticulum (ER). Bcl-xL is a major regulator of cellular apoptotic signalling by expressing Bcl-2 proteins. The Stable Bcl-xL Mouse Embryonic Fibroblast (ER) Cell Line is a useful model in studying apoptosis regulation of tissue differentiation and homeostasis focusing on the specific activities of Bcl-xL at the endoplasmic reticulum as well as how Bcl-xL functions in the regulation of Ca2+ signalling. This cell line is also useful in screening and development of new therapeutic strategies for cancer and other diseases.
