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Biochemie
Imunoanalýza
Stable FUT8 Over-expressing HEK293S GnT I-/- Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Human (H. sapiens) |
| Donor History |
Embryo |
| Growth Properties |
Suspension ,round |
| Growth Conditions |
Grow cells in shaker at 140 rpm/min. PriGrow X Series Medium (TM6010) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. Selection with 1 µg/ml Puromycin (G264) |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/stable-fut8-over-expressing-hek293s-gnt-i-cell-line.html |
Human HEK293S GnT I-/- parental cell line was infected with recombinant lentivirus carrying the FUT8 gene in the pLV-CT vector to establish the Stable FUT8 Over-expressing HEK293S GnT I-/- Cell Line. FUT8 gene encodes for the fucosyltransferase 8 enzyme (α1,6-fucosyltransferase) which is involved with the transfer of fucose from GDP-fucose to N-linked type complex glycopeptides. It has been linked that FUT8 can independently fucosylate high mannose N-glycans without GnTI. Manipulated to over express FUT8, these cells can be utilized for studies relating to fucosylation and the involvement of the enzyme.
