Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
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Třepačky
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Centrifugy
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Real-time PCR cyclery
Fluorometry
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Pipetovací roboty
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Jednokanálové
Multikanálové
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PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
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Intolerance
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Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
Stable HEK293T Expressing Human ECD GC-B (GCB-435) Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Human (H. sapiens) |
| Donor History |
Embryo |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/stable-hek293t-expressing-human-ecd-gc-b-gcb-435-cell-line.html |
The Stable HEK293T Expressing Human ECD GC-B (GCB-435) Cell Line contains the gene encoding for the extracellular ligand-binding domain (ECD) of the human GC-B. The expression of the ECD was done by transfecting HEK293T cells using a bicistronic mammalian expression vector containing the gene for the ECD. This stable cell line is not only convenient for the assessment of various drugs, it is also useful in characterizing other GCase receptors for which physiological functions are yet to be identified as well as for analyzing downstream signalling involving the ECD of the human GC-B.
