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Imunoanalýza
Stable HepG2 Expressing Murine ADH1 (VA-13) Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Liver |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,15 ,White ,Hepatocellular carcinoma |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 400 µg/ml Zeocin for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/stable-hepg2-expressing-murine-adh1-va-13-cell-line.html |
Stable HepG2 Expressing Murine ADH1 (VA-13) Cell Line is recommended for use to study the production of acetaldehyde and its effects in dysfunctions in alcoholic liver disease. The cell line expresses mouse ADH1 (Class 1 Alcohol Dehydrogenase) which oxidizes allyl alcohol to produce toxic acetaldehyde. The Va-13 cells may use used to investigate ethanol metabolism.
