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Stable HsTRPML1 (V446L):YFP Expressing HEK293 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Human (H. sapiens) |
| Donor History |
Embryo |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 600 µg/ml Geneticin/G418 (G271) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/stable-hstrpml1-v446l-yfp-expressing-hek293-cell-line.html |
HEK293 cells were transfected with pcDNA3.1 construct carrying HsTRPML1 (V446L):YFP and were selected under the presence of G418 to create the Stable HsTRPML1 (V446L):YFP Expressing HEK293 Cell Line to study endolysosomal ion channels. Please note that cells may take up to 72 hours to attach post-thaw.
