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Stable RGMb Knockdown PC-3 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Prostate |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,62 ,Caucasian ,Prostate carcinoma |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 500 ng/ml Blasticidin for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/stable-rgmb-knockdown-pc-3-cell-line.html |
Repulsive guidance molecule b (RGMb) belongs to the RGM family. RGMs are known to play an inhibitory role in prostate cancer through the suppression of cell growth, adhesion, migration and invasion. This cell line (Cat. T6327) features a stable knockdown of RGMb in PC-3 cells. These cells show increased Smad-3 activation and reduced levels of Smad-1, alongside ID1 upregulation. Therefore, this cell line is suitable for prostate cancer related research.
